高效液相色谱法检测蔬菜中的吡虫啉残留_张雪辉.pdf

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.4 高效液相色谱法检测蔬菜中的吡虫啉残留 张雪辉，杜雨倩，徐芳，王琦 （昆明医学院公共卫生学院营养与食品研究所，云南昆明呈贡新城 650500） 摘要：本文建立了一种果蔬中吡虫啉农药残留的高效液相色谱检测方法。样品经乙腈提取、盐析、LC-NH2 小柱净化，采用高效 液相色谱仪-紫外检测器进行测定，色谱柱：Waters Symmetry Shield™ C18 （4.6×250 mm ，5 μm ）；柱温：30.0 ℃；检测波长：275 nm； 流动相：甲醇:水: 乙腈=55:25:20 （体积比）；流速：0.8 mL/min；进样量：10.0 µL。吡虫啉在0.1 mg/L 至 1.0 mg/L 的添加范围内线性 良好，线性关系系数 r 为 0.9925；添加水平为 0.05~0.20 mg/kg 时，西红柿样品的平均回收率为 95.4%~106.8%，相对标准偏差为 2.66%~7.99%。结果表明该方法具有快速、灵敏、准确等优点，适合蔬菜中吡虫啉农药残留的测定。 关键词：吡虫啉；高效液相色谱法；农药残留 文章篇号：1673-9078(2012)4-466-468 Determination of Imidaeloprid Pesticide Residue in Fresh Vegetables by HPLC ZHANG Xue-hui, DU Yu-qian, XU Fang, WANG Qi (Nutrition and Food Institute, School of Public Health, Kunming Medical College, Kunming 650500, China) Abstract: A novel high performance liquid chromatography (HPLC) method was developed for the analysis of imidaeloprid pesticide residue in fresh vegetables. Imidaeloprid was extracted from the samples by acetonitrile, purified with LC-NH2 column and determined by high performance liquid chromatography with UV detection. Symmetry Shield™ C column (4.6×250 mm, 5 μm) using the mixture of methanol, 18 water and acetonitrile (55:25:20) as the mobile phase. The column temperature, flow rate，detection wavelength and sample volume were 30.0 ℃, 0.8 mL/min, 275 nm and 10.0 µL, respectively. Within the linear range of 0.1~1.0 mg/L, the correlation coefficient, the recoveries of imidaeloprid in tomato and the relative standard deviations (RSD) were 0.9925, 95.4%~106.8 % and 2.66%~7.99%, respectively. The method was simple, effective and accurate. It can be applied to determinate imidaeloprid