copper transporter 2 content is lower in liver and heart of copper-deficient rats铜转运蛋白2内容是低copper-deficient老鼠的肝脏和心脏.pdf

Int. J. Mol. Sci. 2010, 11, 4741-4749; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Brief Report Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats Jesse Bertinato 1,*, Sébastien Duval 1 and Mary R. L’Abbé2 1 Nutrition Research Division, Health Products and Food Branch, Health Canada, Sir Frederick G. Banting Research Centre, 251 Sir Frederick Banting Driveway, Ottawa, Ontario, K1A 0K9, Canada; E-Mail: drduby@ 2 Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, FitzGerald Building, 150 College Street, Toronto, Ontario, M5S 3E2, Canada; E-Mail: mary.labbe@utoronto.ca * Author to whom correspondence should be addressed; E-Mail: jesse.bertinato@hc -sc.gc.ca; Tel.: +613-957-0924; Fax: +613-941-6182. Received: 20 October 2010; in revised form: 16 November 2010 / Accepted: 17 November 2010 / Published: 19 November 2010 Abstract: Copper (Cu) transporter 2 (Ctr2) is a transmembrane protein that transports Cu across cell membranes and increases cytosolic Cu levels. Experiments using cell lines have suggested that Ctr2 expression is regulated by Cu status. The importance of changes in Ctr2 expression is underscored by recent studies demonstrating that lower Ctr2 content in cells increases the cellular uptake of platinum-containing cancer drugs and toxicity to the drugs. In this study, we examined whether Ctr2 expression is altered by