内源性及外源性一氧化氮对正常及滴注博莱霉素大鼠肺泡巨噬细胞生存的调节.pdf

Acta Physiologica Sinica , October 25, 2005, 57 (5): 619-626 619 Research Paper Modulatory action of endogenous and exogenous nitric oxide on survival of alveolar macrophages from normal and bleomycin-treated rats 1,* 1 2 1 CHEN Xiao-Ling , HUANG Shan-Sheng , LIU Kun , AI Jie 1Department of Pathophysiology, Institute of Basic Medical Sciences; 2Department of Biochemistry, School of Basic Medical Sciences, Hebei Medical University, Shijiazhuang 050017, China Abstract: To investigate the modulatory action of endogenous and exogenous nitric oxide (NO) on survival of alveolar macrophages (AMs) in different cellular states, AMs from normal rats (normal AMs) and from bleomycin (BLM)-treated rats (BLM AMs) were incubated by sodium nitroprusside (SNP, NO donor) and L -arginin (L -Arg, NO precursor), respectively. The survival of AMs was evaluated by apoptosis and cell cycles. The molecular mechanisms were investigated by the contents of Bcl-2, Bax proteins in AMs. The results are as follows: (1) The degree of BLM AMs apoptosis was higher than that of normal AMs; the number of BLM AMs in G /G phases was less than that of normal AMs; there was no significant difference in S+G M phases between the number of BLM 0 1 2 AMs and that of normal AMs. (2) Down-regulation of Bcl-2 and up-regulation of Bax occurred in BLM AMs, compared to those in normal AMs. (3) Apoptosis of AMs, either normal AMs or BLM AMs, was induced by both SNP and L -Arg, when compared to their respective control; only the number of BLM AMs in S+G2M phases was increased by L -Arg. (4) SNP and L -Arg induced a down- regulation of Bcl-2 and an up-regulation of Bax proteins in normal AMs, but did not induce the same change pattern in BLM AMs. (5) The Bax in BLM AMs was down-regulated by