索骨丹提取物的体外抑菌作用.doc

精品论文 参考文献 索骨丹提取物的体外抑菌作用 王晓梅 马琳 俎蕾 雷华 孙冰清 李新凤 马丹 　　[摘 要]目的 研究索骨丹提取物的体外抑菌活性。方法 采用超声法提取索骨丹中蒽醌、多糖、鞣质等主要成分，考察其对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌及铜绿假单胞菌的体外抑菌能力。结果 由最小抑菌浓度判断，铜绿假单胞菌对鞣质和水提物最敏感；金葡菌对蒽醌及鞣质较敏感；枯草杆菌对蒽醌、鞣质及水提物较敏感。由抑菌圈判断蒽醌对枯草杆菌作用较强；鞣质对金葡菌和大肠杆菌作用强；水提物对枯草杆菌作用最强。蒽醌和水提物能明显增加枯草杆菌溶液的电导率。结论 索骨丹提取物对4种致病菌均有抑制作用。 　　[关键词]索骨丹提取物 大肠杆菌 金黄色葡萄球菌 枯草芽孢杆菌 铜绿假单胞菌 　　Bacteriostasis of Rodgersia Aesculifolia Extract in Vitro 　　Wang Xiaomei, Ma Lin, Zu Lei, Lei Hua, Sun Bingqing, LI Xinfeng, Ma Dan 　　Abstract: Objective To investigate the inhibitory effect of antifungal substances extracted from Rodgersia aesculifolia in vitro. Methods Extracting anthraquinone, polysaccharide, tannin and water mixture from rodgersia aesculifolia by ultrasonic. Escherichia coli, staphylococcus aureus, bacillus subtilis and pseudomonas aeruginosa were tested bacteria. Antibacterial activity of 4 kinds of extracts was measured. Results According to the minimum inhibitory concentration, pseudomonas aeruginosa was sensitive to tannin and water extracts, staphylococcus aureus to anthraquinone and tannin, bacillus subtilis to anthraquinone, tannin and water extracts. Bacteriostatic ring was as follow: anthraquinone has strong effect on bacillus subtilis; tannin on staphylococcus aureus and escherichia coli; Anthraquinone and water extract could significantly increase the conductivity of bacillus subtilis solution. Conclusion Rodgersia aesculifolia extracts have inhibitory effect on the above 4 kinds of pathogenic bacteria. 　　Key words: Rodgersia aesculifolia extracts; Escherichia coli; staphylococcus aureus; bacillus subtilis; pseudomonas aeruginosa 　　DOI:10.3760/cma.j.issn.0376-2491.2014.24.023 　　基金项目：陕西省科技发展计划项目（2010K01-201）；宝鸡文理学院重点项目（ZK12013）。 　　作者单位：721013，陕西宝鸡，宝鸡文理学院化学化工学院 　　索骨丹，又名水五龙、慕荷、牛角七、鬼灯檗等。为虎耳草科鬼灯檗属植物老蛇盘Rodgersia aesculifolia Batal.的 根状茎。味苦、涩，性平。功效凉血止血，消肿解毒。用于甲状腺肿，咽喉肿痛，吐血，咯血，崩漏，腹泻，痢疾，便血等。尽管治疗范围广，但主要在秦岭当地被医家应用。1977年被收入于《中国药典》第一部。 其化学成分包括矮茶素，糖类，鞣质，蒽醌，黄酮，淀粉等[1-2]。为开发地方性药材，本实验就索骨丹的几种提取物对大肠杆菌、铜绿假单胞菌