combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo结合cyclooxygenase-1和cyclooxygenase-2选择性抑制剂对卵巢癌的影响癌体内.pdf

Int. J. Mol. Sci. 2011, 12, 668-681; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo Wei Li *, Jie Wang, Hong-Ru Jiang, Xiao-Li Xu, Jun Zhang, Mei-Lin Liu and Ling-Yun Zhai Department of Gynecology, Nanjing Medical University of Hangzhou Hospital, 261 Huansha Road, Hangzhou, Zhejiang 310006, China; E-Mails: hongsheqiao@ (J.W.); hongrujiang@ (H.-R.J.); joyce.xiaolixu@ (X. -L.X.); viola0116@163.com (J.Z.); lm163.com (M.-L.L.); 494610422@ (L.-Y.Z.) * Author to whom correspondence should be addressed; E-Mail: wei5901482@; Tel.: +86-0571 Fax: +86-0571 Received: 30 November 2010; in revised form: 28 December 2010 / Accepted : 10 January 2011 / Published: 18 January 2011 Abstract: The present study was designed to investigate the combined effects of cyclooxygenase (COX)-1 and COX-2 selective inhibitors on human ovarian SKOV-3 carcinoma cells xenograft-bearing mice. The animals were treated with 3 mg/kg SC-560 (a COX-1 selective inhibitor) alone, 25 mg/kg celecoxib (a COX-2 selective inhibitor) alone, or SC-560/celecoxib by gavage, twice a day for three weeks. To test the mechanism of inhibition of tumor growth by COX selective inhibitors, the index of proliferating cells