combinatorial signal integration by apetala2ethylene response factor (erf)-transcription factors and the involvement of ap2-2 in starvation response组合信号集成通过apetala2ethylene响应因子(小块土地)转录因子和饥饿ap2-2参与反应.pdf

Int. J. Mol. Sci. 2012, 13, 5933-5951; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Combinatorial Signal Integration by APETALA2/Ethylene Response Factor (ERF)-Transcription Factors and the Involvement of AP2-2 in Starvation Response Marc Oliver Vogel, Deborah Gomez-Perez, Nina Probst and Karl-Josef Dietz * Biochemistry and Physiology of Plants, Bielefeld University, 33501 Bielefeld, Germany; E-Mails: marc_oliver.vogel@uni-bielefeld.de (M.O.V.); debsgp@ (D.G.-P.); bst@ (N.P.) * Author to whom correspondence should be addressed; E-Mail: karl-josef.dietz@uni-bielefeld.de; Tel.: +49-521-106-5589; Fax: +49-521-106-6039. Received: 15 March 2012; in revised form: 21 April 2012 / Accepted: 8 May 2012 / Published: 16 May 2012 Abstract: Transcription factors of the APETALA 2/Ethylene Response Factor (AP2/ERF)- family have been implicated in diverse processes during development, stress acclimation and retrograde signaling. Fifty-three leaf-expressed AP2/ERFs were screened for their transcriptional response to abscisic acid (ABA), 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), methylviologen (MV), sucrose and high or low light, respectively, and revealed high reactivity to these effectors. Six of them (AP2-2, ARF14, CEJ1, ERF8, ERF11, RAP2.5) were selected for combinatorial response analysis to ABA, DCMU and high light. Additive, s