colorimetric immunoassay for detection of tumor markers比色法检测肿瘤标志物.pdf

Int. J. Mol. Sci. 2010, 11, 5077-5094; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Review Colorimetric Immunoassay for Detection of Tumor Markers Yongmei Yin 1,*, Ya Cao 2, Yuanyuan Xu 2 and Genxi Li 2,3,* 1 Department of Oncology, Jiangsu Province Hospital, Nanjing 210029, China 2 Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China; E-Mails: cone_zimint@ (Y.C.); ladyxuyuanyuan@ (Y.X.) 3 Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China * Authors to whom correspondence should be addressed; E-Mails: ym.yin@ (Y.Y.); genxili@ (G.L.); Tel.: +86-25-681-360-43, +86-25-835-935-96; Fax: +86-25-837-100-40, +86-25-835-925-10. Received: 7 November 2010; in revised form: 24 November 2010 / Accepted: 25 November 2010 / Published: 7 December 2010 Abstract: Tumor markers are substances, usually proteins, produced by the body in response to cancer growth, or by the cancer tissue itself. They can be detected in blood, urine, or tissue samples, and the discovery and detection of tumor markers may provide earlier diagnosis of cancer and improved therapeutic intervention. Colorimetric immunoassays for tumor marker detection have attracted considerable attention, due to their simplicit