cultivable alginate lyase-excreting bacteria associated with the arctic brown alga laminaria可培养的细菌藻酸盐lyase-excreting与北极褐藻类植物.pdf

Mar. Drugs 2012, 10, 2481-2491; doi:10.3390/m OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Cultivable Alginate Lyase-Excreting Bacteria Associated with the Arctic Brown Alga Laminaria Sheng Dong, Jie Yang, Xi-Ying Zhang, Mei Shi, Xiao-Yan Song, Xiu-Lan Chen * and Yu-Zhong Zhang State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China; E-Mails: Ds518@126.com (S.D.); 645670894@ (J.Y.); zhangxiying@ (X.-Y.Z.); clubstone@ (M.S.); xiaoyansong@ (X.-Y.S.); zhangyz@ (Y.-Z.Z.) * Author to whom correspondence should be addressed; E-Mail: cxl0423@; Tel.: +86-531 Fax: +86-531 Received: 15 August 2012; in revised form: 24 September 2012 / Accepted: 26 October 2012 / Published: 6 November 2012 Abstract: Although some alginate lyases have been isolated from marine bacteria, alginate lyases-excreting bacteria from the Arctic alga have not yet been investigated. Here, the diversity of the bacteria associated with the brown alga Laminaria from the Arctic Ocean was investigated for the first time. Sixty five strains belonging to nine genera were recovered from six Laminaria samples, in which Psychrobacter (33/65), Psychromonas (10/65) and Polaribacter (8/65) were the predominant groups. Moreover, 21 alginate lyase-excreting strains were further screened from these Laminaria-associated bacteria. These alginate lyase-excreting strains belong to five genera. Psychromonas