（综述）植物化学成分及其代谢物的定性和定量分析.doc

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry Haifeng Wua, , Jian Guob, , Shilin Chena, , Xin Liuc, , Yan Zhoud, , Xiaopo Zhanga, , Xudong Xua, , a Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, PR China b Department of DMPK, AstraZeneca Pharmaceuticals, 35 Gatehouse Drive, Waltham, MA 02451, USA c Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, PR China d Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China Corresponding author at: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, No.151, Malianwa North Road, Haidian District, Beijing 100193, PR China. Tel.: +86 10 5783 3296; fax: +86 10 5783 3296. Received 14 May 2012 Revised 30 August 2012 Accepted 2 September 2012 Available online 11 September 2012 /10.1016/j.jpba.2012.09.004, How to Cite or Link Using DOI Permissions Reprints View full text Purchase?$31.50 1. Introduction 2. Qualitative analysis 3. Quantitative analysis 4. Application of LC–MS in fingerprinting analysis 5. Conclusions Acknowledgments References Abstract Over the past few years, the applications of liquid chromatography coupled with mass spectrometry (LC–MS) in natural product analysis have been dramatically growing because of the increasingly improved separation and detection capabilities of LC–MS instruments. In particular, novel high-resolution hybrid instruments linked to ultra-high-performance LC and the hyphenations of LC–MS with other separation or analytical techniques greatly aid unequivocal identification and highly sensitive quantification of natural products at trace concentrations in complex matrices. With the aim of providing an up-to-date overview of LC–MS applications on the analysis of plant-derived compounds,