comparison of nasal and bronchial epithelial cells obtained from patients with copd比较鼻和支气管上皮细胞从慢性阻塞性肺病患者.pdf

Comparison of Nasal and Bronchial Epithelial Cells Obtained from Patients with COPD 1,2 1,2. 1 . David M. Comer , J. Stuart Elborn , Madeleine Ennis * 1 Centre for Infection and Immunity, School of Medicine, Dentistry and Biomedical Sciences, The Queen’s University of Belfast, Belfast, United Kingdom, 2 Respiratory Department, Belfast City Hospital, Belfast, United Kingdom Abstract For in vitro studies of airway pathophysiology, primary epithelial cells have many advantages over immortalised cell lines. Nasal epithelial cells are easier to obtain than bronchial epithelial cells and can be used as an alternative for in vitro studies. Our objective was to compare nasal and bronchial epithelial cells from subjects with COPD to establish if these cells respond similarly to pro-inflammatory stimuli. Cell cultures from paired nasal and bronchial brushings (21 subjects) were incubated with cigarette smoke extract (CSE) prior to stimulation with Pseudomonas aeruginosa lipopolysaccharide. IL-6 and IL-8 were measured by ELISA and Toll-like receptor 4 (TLR-4) message and expression by RT-PCR and FACS respectively. IL-8 release correlated significantly between the two cell types. IL-6 secretion was significantly less from bronchial compared to nasal epithelial cells and secreted concentrations did not correlate. A 4 h CSE incubation was immunosuppressive for both nasal and bronchial cells, however prolonged incubation for 24 h was pro-inflammatory solely for the nasal cells. CSE reduced TLR-4 expression in bronchial cells only after 24 h, and was without effect on mRNA expression. In subjects with COPD, nasal epithelial cells cannot substitute for in vitro bronchial epithelial cells in airway inflammation studies. Citation: Comer DM, Elborn JS, Ennis M (2012) Comparison of Nasal and Bron