基于MSP430F435单片机的血糖仪设计（The design of glucose meter based on MSP430F435 single chip microcomputer）.doc

基于MSP430F435单片机的血糖仪设计（The design of glucose meter based on MSP430F435 single chip microcomputer） Preparation of culture medium and microbiological inoculation technique [applicable objects] environmental science major [experimental study] 4 Preparation of medium One, the purpose of the experiment 1. Understand the main components of artificial culture medium and general principles of preparation. 2. Master the manufacturing method and process of basic culture medium. 2. Experimental principle Culture medium is an artificial nutrient matrix suitable for microbial growth and propagation. The media should have the following conditions: to have suitable nutrients and water; Having a suitable PH value; After the preparation, sterilization should be sterilized. The media can be divided into liquid medium, solid medium and semi-solid medium according to physical state. Use AGAR as a coagulant. Basic procedures for preparation of culture medium: ingredients, solutization, determination and correction of PH, filtration, separation, sterilization and aseptic testing Iii. Instruments and equipment Test tube, graduated straw, gauze, filter paper, triangle bottle, culture dish, measuring tube, funnel, cotton plug, electric furnace, balance, wrapping paper, cotton thread, ph test paper, glass rod. Reagent: beef paste, peptone, sodium chloride, dipotassium phosphate, AGAR bar, 0.1 mol/L and 1mol/L hydroxide, 0.1 mol/L and 1 mol/L of hydrochloric acid solution, distilled water, etc. 4. Relevant knowledge points I. preparation principles and types (1) principle of preparation of culture medium 1. Culture medium: the medium is a substrate for the nutrition and specific environmental conditions needed for the growth and development of edible fungi, such as mushroom fungus, which is a kind of grass saprophytes in organic nutrition. Preparation of culture medium to consider not only what it needs of carbon-containing compounds, nitrogen compounds, mineral salts, growth factors, but also