unique interplay between sugar and lipid in determining the antigenic potency of bacterial antigens for nkt cells独特的糖和脂质之间的相互作用在决定细菌抗原的抗原效价nkt细胞.pdf

Unique Interplay between Sugar and Lipid in Determining the Antigenic Potency of Bacterial Antigens for NKT Cells 1. 1. 1 2,3 4 1 5 Enrico Girardi , Esther Dawen Yu , Yali Li , Norihito Tarumoto , Bo Pei , Jing Wang , Petr Illarionov , 2 4 1 Yuki Kinjo , Mitchell Kronenberg , Dirk M. Zajonc * 1 Division of Cell Biology, La Jolla Institute for Allergy Immunology, La Jolla, California, United States of America, 2 Department of Chemotherapy and Mycoses, National Institute of Infectious Diseases, Tokyo, Japan, 3 Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan, 4 Division of Developmental Immunology, La Jolla Institute for Allergy Immunology, La Jolla, California, United States of America, 5 School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom Abstract Invariant natural killer T (iNKT) cells are an evolutionary conserved T cell population characterized by features of both the innate and adaptive immune response. Studies have shown that iNKT cells are required for protective responses to Gram- positive pathogens such as Streptococcus pneumoniae, and that these cells recognize bacterial diacylglycerol antigens presented by CD1d, a non-classical antigen-presenting molecule. The combination of a lipid backbone containing an unusual fatty acid, vaccenic acid, as well as a glucose sugar that is weaker or not stimulatory when linked to other lipids, is required for iNKT cell stimulation by these antigens. Here we