taxonomic identity of the invasive fruit fly pest, bactrocera invadens concordance in morphometry and dna barcoding分类的身份入侵果蝇虫害,bactrocera invadens和谐形态测量学和dna条码技术.pdf
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Taxonomic Identity of the Invasive Fruit Fly Pest,
Bactrocera invadens: Concordance in Morphometry and
DNA Barcoding
1,2 3 1 1 4
Fathiya M. Khamis *, Daniel K. Masiga , Samira A. Mohamed , Daisy Salifu , Marc de Meyer ,
Sunday Ekesi1
1 African Fruit Fly Programme, International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya, 2 Department of Biochemistry and Biotechnology, Kenyatta
University, Nairobi, Kenya, 3 Molecular Biology and Biotechnology Department, International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya, 4 Royal
Museum for Central Africa, Tervuren, Belgium
Abstract
In 2003, a new fruit fly pest species was recorded for the first time in Kenya and has subsequently been found in 28
countries across tropical Africa. The insect was described as Bactrocera invadens, due to its rapid invasion of the African
continent. In this study, the morphometry and DNA Barcoding of different populations of B. invadens distributed across the
species range of tropical Africa and a sample from the pest’s putative aboriginal home of Sri Lanka was investigated.
Morphometry using wing veins and tibia length was used to separate B. invadens populations from other closely related
Bactrocera species. The Principal component analysis yielded 15 components which correspond to the 15 morphometric
measurements. The first two principal axes contributed to 90.7% of the total variance and showed partial separation of
these populations. Canonical discriminant analysis indicated that only the first five canonical variates were statistically
significant. The first two canonical variates contributed a total of 80.9% of the total variance clusterin
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