structural dna nanotechnology from design to applications结构dna纳米技术从设计到应用程序.pdf

Int. J. Mol. Sci. 2012, 13, 7149-7162; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Review Structural DNA Nanotechnology: From Design to Applications Reza M. Zadegan 1,* and Michael L. Norton 2,* 1 Center for DNA Nanotechnology (CDNA), Interdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus C, Denmark 2 Department of Chemistry, Marshall University, Huntington, WV 25755, USA * Authors to whom correspondence should be addressed; E-Mails: reza@inano.au.dk or admin@ (R.M.Z.); norton@ (M.L.N.); Tel.: +45-8715-4959 (R.M.Z.); +1-304-696-3489 (M.L.N.); Fax: +45-8715-4041 (R.M.Z.); +1-304-696-3243 (M.L.N.). Received: 2 May 2012; in revised form: 29 May 2012 / Accepted: 4 June 2012 / Published: 11 June 2012 Abstract: The exploitation of DNA for the production of nanoscale architectures presents a young yet paradigm breaking approach, which addresses many of the barriers to the self-assembly of small molecules into highly-ordered nanostructures via construct addressability. There are two major methods to construct DNA nanostructures, and in the current review we will discuss the principles and some examples of applications of both the tile-based and DNA origami methods. The tile-based approach is an older method that provides a good tool to construct small and simple structures, usually with multiply r