rpl31_sirna对人胰腺癌bxpc_3细胞裸鼠皮下移植瘤生长的抑制作用.pdf

® 2012 10 18 ; 20(29): 2782-2788 wcjd@ ISSN 1009-3079 (print) ISSN 2219-2859 (online) BASIC RESEARCH pancreatic cancer. , METHODS: The siRNA sequence targeting to , RNA human RPL31 gene was designed. Human pan- creatic cancer BxPC-3 cells were subcutaneously inoculated into Balb/c nude mice to develop , RNA a transplantation tumor model of human pan- creatic cancer. According to the tumor volume, nude mice were randomly divided into three groups: vehicle control group, negative control group and RPL31-siRNA injection group. Mice . of the RPL31-siRNA injection group were in- tratumorally injected with RPL31-siRNA using RNAi-Mate. Tumor volumes were calculated daily. On the 16th day after the first intratu- moral injection, all the mice were sacrifi ced and SiRNA-mediated RPL31 gene the tumors were taken out to undergo real-time silencing inhibits the growth