(NCBI如何查找序列.docx

请练习在GenBank获得一个GenBank序列文件，解释以一下各个部分代表的含义。开Genbank主页（/Web/Genbank/），②database选择“Nucleotide”，输入“Monkey（猴子）“，点击“Search”单击“search”后得到以下页面：然后选择第一个序列文献打开；找到如下图的位置，选择“file“，保存到电脑。序列内容如下：序列内容各部分意思如下：/nuccore/K01786.1Go to:/nuccore/K01786.1/nuccore/K01786.1/nuccore/K01786.1LOCUS AGMSV40RP436 bp DNA linear PRI （名称） （碱基数） （分子类型） （分子类型） （灵长类）27-APR-1993（修正日期）DEFINITION African green monkey SV40 homologue replication origin.（定义行，精要描述序列特征）ACCESSION K01786（检索号）VERSIONK01786.1 GI:176550（版本信息）KEYWORDS origin of replication.（关键词）SOURCEChlorocebus aethiops (Cercopithecus aethiops)（物种来源及常用名）ORGANISM /Taxonomy/Browser/wwwtax.cgi?id=9534Chlorocebus aethiops（科学命名及该物种的分类地位） Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Cercopithecidae; Cercopithecinae; Chlorocebus.REFERENCE1 (bases 1 to 436)（参考文献）AUTHORSQueen,C., Lord,S.T., McCutchan,T.F. and Singer,M.F. （作者）TITLE Three segments from the monkey genome that hybridize to simian （题目） virus 40 have common structural elements JOURNAL Mol. Cell. Biol. 1 (12), 1061-1068 (1981)（期刊来源）PUBMED/pubmed/62872166287216（数据库编号）REFERENCE2 (sites) （参考文献） AUTHORSDynan,W.S., Saffer,J.D., Lee,W.S. and Tjian,R. （作者） TITLE（题目） Transcription factor Sp1 recognizes promoter sequences from the monkey genome that are simian virus 40 promoter JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82 (15), 4915-4919 (1985)（期刊来源） PUBMED/pubmed/29918982991898（数据库编号）COMMENT（注释） Original source text: African green monkey liver DNA, clone pCaOri7.01 [1]. [2] sites; Sp1 binding sites. Draft entry and clean copy sequences for [2] kindly provided by W.Dynan, 31-OCT-1985. Two segments of monkey DNA were hybridized and compared to SV40 DNA. Each contained multiple copies of the sequence gggcggrr which also appears six times near the origin of SV40 [1]. Both contained a long internal degenerate repeat [1]. The SV40 origin of replication contains several long repeats [1]. The SV40 hyb