specific eradication of hiv-1 from infected cultured cells根除特定的hiv - 1感染培养的细胞.pdf

Levin et al. AIDS Research and Therapy 2010, 7:31 /content/7/1/31 SHORT REPORT Open Access Specific eradication of HIV-1 from infected cultured cells 1 2 2 1* Aviad Levin , Zvi Hayouka , Assaf Friedler , Abraham Loyter Abstract A correlation between increase in the integration of Human Immunodeficiency virus-1 (HIV-1) cDNA and cell death was previously established. Here we show that combination of peptides that stimulate integration together with the protease inhibitor Ro 31-8959 caused apoptotic cell death of HIV infected cells with total extermination of the virus. This combination did not have any effect on non-infected cells. Thus it appears that cell death is promoted only in the infected cells. It is our view that the results described in this work suggest a novel approach to specifi- cally promote death of HIV-1 infected cells and thus may eventually be developed into a new and general anti-viral therapy. Findings numerous copies of unintegrated viral cDNA [22,24]. No cure or vaccine are currently available for the Recently we have shown that this restriction is due to human immunodeficiency virus type 1 (HIV-1) infection inhibition of the viral IN activity as well as of its nuclear as well as for the resulting Acquired Immunodeficiency import by an early expressed viral Rev protein following syndrome (AIDS) [1]. However, an highly active antire- Rev-IN interaction [25-30]. Disruption of the Rev-IN troviral therapy (HAART), which blocks the activities of complex by IN-derived cell permeable peptides, such as the viral reverse transcriptase and protease and i