surface modification on acoustic wave biosensors for enhanced specificity表面改性对声波增强特异性的生物传感器.pdf
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Sensors 2012, 12, 12317-12328; doi:10.3390/s120912317
OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Article
Surface Modification on Acoustic Wave Biosensors for
Enhanced Specificity
Onursal Onen †, Asad A. Ahmad †, Rasim Guldiken and Nathan D. Gallant *
Department of Mechanical Engineering, University of South Florida, 4202 E Fowler Ave, ENB 118,
Tampa, FL 33620, USA; E-Mails: onursalonen@ (O.O.);
aaahmad@ (A.A.A.); guldiken@ (R.G.)
† These authors contributed equally to this work.
* Author to whom correspondence should be addressed; E-Mail: ngallant@;
Tel.: +1-813-974-5840; Fax: +1-813-974-3539.
Received: 2 July 2012; in revised form: 3 September 2012 / Accepted: 4 September 2012 /
Published: 10 September 2012
Abstract: Changes in mass loading on the surface of acoustic biosensors result in output
frequency shifts which provide precise measurements of analytes. Therefore, to detect a
particular biomarker, the sensor delay path must be judiciously designed to maximize
sensitivity and specificity. B-cell lymphoma 2 protein (Bcl-2) found in urine is under
investigation as a biomarker for non-invasive early detection of ovarian cancer. In this
study, surface chemistry and biofunctionalization approaches were evaluated for their
effectiveness in presenting antibodies for Bcl-2 capture while minimizing non-specific
protein adsorption. The optimal combination of seque
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