不同产地石斛属种质资源的ISSR遗传多样性分析.PDF

不同产地石斛属种质资源的 ISSR 遗传多样性分析 卢家仕1, 2, 3，卜朝阳1*，吕维莉2，苏建睦3，黄昌艳1，李春牛1 1. 广西农业科学院花卉研究所，广西 南宁 530007 2. 广西农业科学院 广西作物遗传改良生物技术重点开放实验室，广西 南宁 530007 3. 广西大学 农学院，广西 南宁 530004 摘 要：目的 对 24 份不同产地石斛属样品的遗传多样性和亲缘关系进行分析。方法 采用 ISSR 分子标记技术和非加权 平均距离法（UPGMA）对 24 份石斛属样品进行遗传多样性和聚类分析。结果 从 100 条 ISSR 引物中共筛选出 6 条多态性 稳定、清晰的引物， 24 份石斛样品共扩增出 847 个 DNA 片段，平均每个引物扩增出 141 个 DNA 片段，其多态性为 100%。 结论 24份不同产地石斛样品被划分为 6 个类群，遗传多样性非常丰富。 关键词：石斛属；种质资源；ISSR 分子标记；遗传多样性；亲缘关系 中图分类号：R282.12 文献标志码：A 文章编号：0253 - 2670(2013)01 - 0 - 0 ISSR analysis on genetic diversity of germplasms resources in Dendrobium SW. from different habitats LU Jia-shi1, 2, 3, BU Zhao-yang1, LV Wei-li2, SU Jian-mu3, HUANG Chang-yan1, LI Chun-niu1 1. Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China 2. Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, China 3. College of Agronomy, Guangxi University, Nanning 530004, China Abstract: Objective To analyze the genetic diversity and genetic relationship of 24 plant samples in Dendrobium SW. from different habitats. Methods The molecular marker technique of inter-simple sequence repeat (ISSR) and unweighted pair-group method with arithmetic means (UPGMA) were used to study the genetic diversity and cluster analysis of 24 samples. Results Six primers with stable and clear diversity were sieved from 100 ISSR primers. DNA fragments (847) were amplified from 24 samples, and 141 DNA fragments were amplified from each primer in average, with 100% of polymorphic bands. Conclusion Th