常见重组蛋白的纯化.PDF
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11
1 2
3 4
6XHis pH
GST 10-20mM
MBP
A IgG pH
Flag peptide Flag ,M1,M2 pH EDTA
Poly-Arg SP
Poly-Cys DTT
Poly-Phe
EGTA
GST
2
His-Tag
IMAC
21 IMAC(Immobilized Metal-ion affinity chromatography)Porath et al.1975
IDA
1987 Smith et al.
IDA-sephadex G-25 1986
2+
Ni -IDA-sephadex G-25 1987
Hochuli et al.Ni2+-NTA1988
IMAC
IMAC
1986 Porath et al.Fe3+-IDA-sephadex G-25
Ga3+-IDA
IMAC
IMAC
IDA
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