离体家兔肠肌运动.doc

家兔离体肠肌活动的影响实验 周瑶瑶 （浙江中医药大学滨江学院 2009临床医学3班，20092270126） 【摘要】目的 研究0.01%乙酰胆碱（Ach）、0.01%肾上腺素（E）、1NHCl、1NNaOH溶液25℃台氏液及缺氧等条件对家兔离体肠肌运动的影响。方法 制备家兔离体小肠平滑肌标本，放置于灌流浴槽中，生物信号采集处理系统记录在不同因素作用下张力曲线。 结果 滴加0.01%乙酰胆碱（Ach）、1NNaOH溶液后，肠肌运动张力和频率都增大；滴加0.01%肾上腺素（E）、1NHCl、肠肌运动张力和频率迅速降低；25℃台氏液刺激离体肠肌时，肠肌运动张力和频率先迅速下降后逐渐增大；停止通氧后，肠肌运动张力和频率逐渐降低。结论 0.01%乙酰胆碱（Ach）、1NNaOH溶液刺激能促进肠肌运动，0.01%肾上腺素（E）、1NHCl能抑制肠肌运动，短时低温能使肠肌运动稍降后又加强直至正常，缺氧使肠肌运动减弱。 关键词：Hcl、NaoH、离体肠肌、乙酰胆碱???肾上腺素 Rabbit intestinal muscle activity from the body of the impact experiment Zhou yao-yao (zhejiang university of traditional Chinese medicine binjiang college class 3, 2009 clinical medicine 20092270126) Abstract : objective Research 0.01% acetylcholine (Ach), 0.01% epinephrine (E), 1NHCl, 1NNaOH solution ℃ Taiwans fluid and 25 of rabbit oxygen from the body condition such as the influence of intestinal muscle movement.. Methods Preparation vitro intestinal smooth muscle specimens rabbit, placed in the perfusion bath slot biological signal acquisition and processing system records in different factors under the role of tension curve. Results Drops of add 0.01% acetylcholine (Ach), 1NNaOH solution intestinal muscle movement, tension and frequency increases; Drops of add adrenaline 0.01% (E), 1NHCl, intestinal muscle movement tension and frequency rapidly reduces; 25 ℃ Taiwans fluid from the body when the stimulus, intestinal muscle tension and frequency of intestinal muscle movement first fell rapidly after gradually increases; Stop after the intestinal muscle movement, oxygen tension and frequency gradually reduced. Conclusion 0.01% acetylcholine (Ach), 1NNaOH solution stimulation can promote intestinal muscle movement, 0.01% epinephrine (E), 1NHCl can restrain bowel muscle movement, short-term low temperature can make after lower intestinal muscle movement, and strengthen until normal oxygen made intestinal muscle motion decreases. Keywords Hydrochloric acid；Sodium hydroxide；Isolated small intestine; acetylcholine; adrenaline 消化道平滑肌