DB37_T 3756-2019肥料中10种植物生长调节剂的高通量快速筛选　液相色谱-质谱联用法.pdf

ICS65. 080G 20DB37山 長东省地方标准DB37/T 3756—2019肥料中10种植物生长调节剂的高通量快速筛选液相色谱-质谱联用法High-throughout screening of ten growth regulators in fertilizers by liquidchromatography-tandem mass spectometr布2020-01-05实施山东省市场监督管理局发布 DB37/T 3756—2019目次前言111范围.2规范性引用文件3原理4试剂和材料5仪器.6分析步骤6.1样品制备6.2提取6.3测定.6.4空白试验6.5结果表述6.6允许差.附录A（资料性附录）10种植物生长调节剂信息一览表，附录B（资料性附录）标准物质的质谱分析参考参数（资料性附录）标准物质的总离子流质谱图和提取离子质谱图 DB37/T 3756—2019附录C（资料性附录）标准物质的总离子流质谱图和提取离子质谱图标准物质的总离子流质谱图见图C.1。200ng/mlhunbiaoL94.29 4.572: MRM of 6 Channels ES+100TIC1.02e703.932.004.006.008.00hunbiaoLg1:MRMof14Channels ES-1003.59TIC%4.031.29e503.16Time2.004.006.008.00图C.1标准物质的总离子流质图 DB37/T 3756—2019标准物质（赤需酸、脱落酸、2，4-D、兼乙酸）的提取离子质谱图见图C.2。200ng/mlhunbiaoLg1:MRMof14ChannelsEs-10033.17345.03239.06 (chlmelsuan2)-10 .471.922.064.288.119.35.9.944382.004.006.001:MRM of14ChannelsES-8.00hunbiaoLg10033.1 6345.03143.06 (chimeisuan1)6.35e303.672.004.006.001:MRM of14ChannelsEs.8.00hunbiaoLg10073.56263.11219.07(tuolu0suan2)3.343,610-3pip4.982.004.006 .008.00hunbiaoLg3.551:MRMof14ChannelsES.1007263.11152.93(tuolu0suan1).36e42.004.006.008.00hunbiaoLg1:MRM of14 ChannelsES-10034.0 3219160.8 (24D1)4.04e49.05,2.004.006.008.00hunbiaoLg:MRM of14ChannelsES.10074 9(24D2)5.40e32.004.006.008.00hunbiaoL93.901:MRMof14ChannelsES.1003185141(nalyisuan1)5.826.386,73 7.829681.62e31.37-1.652.58.9.612.004.006.008.00图C.2标准物质（赤霉酸、脱落酸、2.4-D、乙酸）的提取离子质谱图9 DB37/T准物质（多效唑、烯效唑、氧吡腺、5-硝基邻甲氧基苯酚钠、2-硝基苯酚钠、4-硝基苯酚钠）的提取离子质谱图见图C.3。200ng/mlhunbiaols 0 0 1hunbiaoLg4.0 0.001 D 0 -4 2 94,57hunblaoLs0 0.0 10074 .5 72: MR.0 0.0hunbiaoLs4.57 .0 00.0° 3.952.3 4 ± 5hunbisol90 g,4 .0 06 .0 08003 .9 3200ng/mlh un b isolg3 .5 94.1504hunbisoLs2.004.003 .0 01:MRM1003168.15123.12(5NO2)lof14ChannehES3.57,02.3 .6 52.0 04.0 06.008.00h un b is oLs100-73.57I:MRM138.15108.12(2NO13.9064hunbiaoLs2.0 04.0 06 .0 0I:MRM8.0010033.57138.1592.18(2N02l4ChanneES2be32 .0 04.0 06.000 0.8图C.3标准物质（多效唑、烯效唑、氯吡脲、5-硝基邻甲氧基苯酚钠、2-硝基苯酚钠、4-硝基苯酚钠）的提取离子质谱图10 DB37/T 3756—2019前言本标准按照GB/T1.1—2009给出的规则起草，本