the expanding role of electrospray ionization mass spectrometry for probing reactive intermediates in solution电喷雾质谱的扩大作用探索活性中间体的解决方案.pdf

Molecules 2012, 17, 11507-11537; doi:10.3390/molecules171011507 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Review The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in Solution Weitao Zhu 1,†, Yu Yuan 2,†, Peng Zhou 1, Le Zeng 1, Hua Wang 1, Ling Tang 1, Bin Guo 1,* and 1 Bo Chen 1 Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), Hunan Normal University, 36 Lushan Road, Changsha 410081, China; E-Mails: weitao0329@126.com (W.Z.); zhou_peng@189.cn (P.Z.); zengle8899@126.com (L.Z.); hiction@ (H.W.); tanya051423@ (L.T.); dr-chenpo@ (B.C.) 2 School of Pharmaceutical Sciences, Central South University, 172 Tongzipo Road, Changsha 410013, China; E-Mail: csuyy@ † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: binnguo@126.com; Tel.: +86-731-8886-5515; Fax: +86-731-8887-2046. Received: 25 July 2012; in revised form: 29 August 2012 / Accepted: 5 September 2012 / Published: 27 September 2012 Abstract: Within the past decade, electrospray ionization mass spectrometry (ESI-MS) has rapidly occupied a prominent position for liquid-phase mechanistic studies due to its intrinsic advantages allowing for efficient “fishing” (rapid, sensitive, specific and simultaneous detection/identification) of multiple intermediates and products directly from a “real-world” solution. In this review we attempt to offer a comprehensiv