stress-induced survival strategies enable salmonella enteritidis to persistently colonize the chicken oviduct tissue and cope with antimicrobial factors in egg white a hypothesis to explain a pandemic应激的生存策略使肠炎沙门氏菌长期殖民鸡输卵管组织和应对抗菌因子蛋白假说来解释大流行.pdf
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Van Immerseel Gut Pathogens 2010, 2:23
/content/2/1/23
HYPOTHESIS Open Access
Stress-induced survival strategies enable Salmonella
Enteritidis to persistently colonize the chicken
oviduct tissue and cope with antimicrobial factors
in egg white: A hypothesis to explain a pandemic
Filip Van Immerseel
Abstract
Background: Egg-associated transmission to humans seems to be characteristic of the Salmonella serotype
Enteritidis, explaining why this particular serotype has caused a worldwide pandemic since the mid ‘80s. Salmonella
Enteritidis is much more capable to persistently colonize the laying hen reproductive tract and to survive in the
hostile egg white, as compared to other serotypes.
Presentation of the hypothesis: It is hypothesized that stress-induced survival mechanisms enable the serotype
Enteritidis to persistently colonize the oviduct without causing damage and excessive inflammation, and to cope
with the antimicrobial compounds present in egg white.
Testing the hypothesis: To test the hypothesis first of all Salmonella Enteritidis genes that are essential for
colonization of the oviduct and survival in eggs need to be identified. Comparative genomics tools should be used
to identify genes or pathogenicity islands that are present in Salmonella Enteritidis and not in the multiple non
egg-contaminating serotypes. High-throughput signature-tagged-mutagenesis approaches, coupled to micro-array
detection of the genes that lead to an attenuated phenotype when mutated is proposed as an ideal tool to
identify genes involved in oviduct colonization and egg white survival. Identifying the stressors and antibacterial
molecules in the oviduct and in the egg white that limit colonization or survival of non-Enteritidis serotypes is a
second important objective that can theoretically be achieved using screenin
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