the evolution of the bacterial luciferase gene cassette (lux) as a real-time bioreporter细菌荧光素酶基因的进化磁带(勒克斯)作为实时bioreporter.pdf

Sensors 2012, 12, 732-752; doi:10.3390/s120100732 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review The Evolution of the Bacterial Luciferase Gene Cassette (lux) as a Real-Time Bioreporter 1 2 2 2 2 2 Dan Close , Tingting Xu , Abby Smartt , Alexandra Rogers , Robert Crossley , Sarah Price , Steven Ripp 2 and Gary Sayler 1,2,* 1 Oak Ridge National Laboratory, The Joint Institute for Biological Sciences, 676 Dabney Hall, Knoxville, TN 37996, USA; E-Mail: dclose@ 2 The Center for Environmental Biotechnology,The University of Tennessee, 676 Dabney Hall, Knoxville, TN 37996, USA; E-Mails: txu2@ (T.X.); asmartt@ (A.S.); aroger24@ (A.R.); rcrossl1@ (R.C.); sprice12@ (S.P.); saripp@ (S.R.) * Author to whom correspondence should be addressed; E-Mail: sayler@; Tel.: +1-865-974-8080; Fax: +1-865-974-8086. Received: 26 November 2011; in revised form: 30 December 2011 / Accepted: 9 January 2012 / Published: 11 January 2012 Abstract: The bacterial luciferase gene cassette (lux) is unique among bioluminescent bioreporter systems due to its ability to synthesize and/or scavenge all of the substrate compounds required for its production of light. As a result, the lux system has the unique ability to autonomously produce a luminescent signal, either continuously or in response to the presence of a specific trigger, across a wide array of organismal hosts.