association of polymorphism in pri-micrornas-371-372-373 with the occurrence of hepatocellular carcinoma in hepatitis b virus infected patients多态性协会pri -小分子核糖核酸- 371 - 372 - 373与肝细胞癌的发生在乙型肝炎病毒感染的患者.pdf

Association of Polymorphism in Pri-microRNAs-371-372- 373 with the Occurrence of Hepatocellular Carcinoma in Hepatitis B Virus Infected Patients 1 1 1 1 1 1 Min-Sun Kwak , Dong Hyeon Lee , Yuri Cho , Eun-Ju Cho , Jeong-Hoon Lee , Su Jong Yu , Jung- 1 1 1 2 2 3 Hwan Yoon , Hyo-Suk Lee , Chung Yong Kim , Jae Youn Cheong , Sung Won Cho , Hyoung Doo Shin , Yoon Jun Kim1* 1 Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea, 2 Department of Gastroenterology, Ajou University School of medicine, Suwon, Republic of Korea, 3 Department of Life Science, Sogang University, Seoul, Republic of Korea Abstract Background: Micro RNAs-371-372-373 (miRNAs-371-373), originating from the same pri-miRNA transcript, are reported to be upregulated in hepatocellular carcinoma (HCC) and to be related to the regulation of hepatitis B virus (HBV) infection. Our study investigated whether pri-miRNAs-371-373 polymorphisms are associated with the risk of HCC occurrence and HBV clearance. Methods: Genetic variations were identified through direct DNA sequencing using TaqMan assay. Three sequence variants of pri-miRNAs-371-373 were identified. Genetic associations of those with HCC occurrence and HBV clearance among patients with HBV infection were analyzed using logistic regression analyses with adjustment for age and gender (n = 1439). Results: For the occurrence of HCC, polymorphism rs3859501C.A acted as a protective factor both in chronic carriers (OR = 0.75, P = 0.005 in a codominant model; OR = 0.71, P = 0.02 in a dominant