α-tocopherol at nanomolar concentration protects pc12 cells from hydrogen peroxide-induced death and modulates protein kinase activitiesα-tocopherol在摩尔浓度从氢peroxide-induced保护pc12细胞死亡和调节蛋白激酶活动.pdf

Int. J. Mol. Sci. 2012, 13, 11543-11568; doi:10.3390/ijms130911543 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article α-Tocopherol at Nanomolar Concentration Protects PC12 Cells from Hydrogen Peroxide-Induced Death and Modulates Protein Kinase Activities Irina O. Zakharova, Tatyana V. Sokolova, Liubov V. Bayunova, Yulia A. Vlasova, Maria P. Rychkova and Natalia F. Avrova * Department of Comparative Neurochemistry, Institute of Evolutionary Physiology and Biochemistry of Russian Academy of Sciences, Thorez avenue, 44, Saint-Petersburg 194223, Russia; E-Mails: zakhar@iephb.ru (I.O.Z.); sokolt1956@mail.ru (T.V.S.); bayunoval@mail.ru (L.V.B.); yousia@mail.ru (Y.A.V.); involved@mail.ru (M.P.R.) * Author to whom correspondence should be addressed; E-Mail: avrova@iephb.ru; Tel.: +7-812-552-7901; Fax: +7-812-552-3012. Received: 9 July 2012; in revised form: 23 August 2012 / Accepted: 4 September 2012 / Published: 14 September 2012 Abstract: The aim of this work was to compare protective and anti-apoptotic effects of α-tocopherol at nanomolar and micromolar concentrations against 0.2 mM H O -induced 2 2 toxicity in the PC12 neuronal cell line and to reveal protein kinases that contribute to α-tocopherol protective action. The protection by 100 nM α