successful reconstruction of tooth germ with cell lines requires coordinated gene expressions from the initiation stage成功的重建牙胚的细胞株需要协调基因表达式从起始阶段.pdf

Cells 2012, 1, 905-925; doi:10.3390/cells1040905 OPEN ACCESS cells ISSN 2073-4409 /journal/cells Article Successful Reconstruction of Tooth Germ with Cell Lines Requires Coordinated Gene Expressions from the Initiation Stage Akihiko Komine and Yasuhiro Tomooka * Department of Biological Science Technology, Science University of Tokyo, 2641 Yamazaki Noda, Chiba 278-8510, Japan * Author to whom correspondence should be addressed; E-Mail: tomoylab@rs.noda.tus.ac.jp; Tel.: +81-4-7122-9697; Fax: +81-4-7125-1841. Received: 17 August 2012; in revised form: 28 September 2012 / Accepted: 24 October 2012 / Published: 30 October 2012 Abstract: Tooth morphogenesis is carried out by a series of reciprocal interactions between the epithelium and mesenchyme in embryonic germs. Previously clonal dental epithelial cell (epithelium of molar tooth germ (emtg)) lines were established from an embryonic germ. They were odontogenic when combined with a dental mesenchymal tissue, although the odontogenesis was quantitatively imperfect. To improve the microenvironment in the germs, freshly isolated dental epithelial cells were mixed with cells of lines, and germs were reconstructed in various combinations. The results demonstrated that successful tooth construction depends on the mixing ratio, the age of dental epithelial c