blunted neuronal calcium response to hypoxia in naked mole-rat hippocampus钝化在裸鼢鼠缺氧海马神经元钙响应.pdf

Blunted Neuronal Calcium Response to Hypoxia in Naked Mole-Rat Hippocampus 1 1,2,3 5 1,2 . 4,6. Bethany L. Peterson , John Larson , Rochelle Buffenstein , Thomas J. Park * , Christopher P. Fall 1 Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois, United States of America, 2 Laboratory of Integrative Neuroscience, University of Illinois at Chicago, Chicago, Illinois, United States of America, 3 Psychiatric Institute, Department of Psychiatry, University of Illinois at Chicago, Chicago, Illinois, United States of America, 4 Department of BioEngineering, University of Illinois at Chicago, Chicago, Illinois, United States of America, 5 Barshop Institute and Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America, 6 Department of Computer Science, Georgetown University, Washington, D. C., United States of America Abstract Naked mole-rats are highly social and strictly subterranean rodents that live in large communal colonies in sealed and chronically oxygen-depleted burrows. Brain slices from naked mole-rats show extreme tolerance to hypoxia compared to slices from other mammals, as indicated by maintenance of synaptic transmission under more hypoxic conditions and three fold longer latency to anoxic depolarization. A key factor in determining whether or not the cellular response to hypoxia is reversible or leads to cell death may be the elevation of intracellular calcium concentration. In the present study, we used fluorescent imaging techniques to measure relative intracellular calcium changes in CA1 pyramidal cells of hippocampal slices during hypoxia. We found that calcium accumulation d