cell-sized liposomes and droplets real-world modeling of living cellscell-sized活细胞的脂质体和水滴现实世界建模.pdf

Materials 2012, 5, 2292-2305; doi:10.3390/ma5112292 OPEN ACCESS materials ISSN 1996-1944 /journal/materials Review Cell-Sized Liposomes and Droplets: Real-World Modeling of Living Cells Tsutomu Hamada 1,* and Kenichi Yoshikawa 2,* 1 School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1, Asahidai, Nomi, Ishikawa 923-1292, Japan 2 Faculty of Life and Medical Sciences, Doshisha University, 1-3, Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan * Authors to whom correspondence should be addressed; E-Mails: t-hamada@jaist.ac.jp (T.H.); keyoshik@mail.doshisha.ac.jp (K.Y.); Tel./Fax: +81-761-51-1670 (T.H.); +81-774-65-6243 (K.Y.). Received: 18 October 2012; in revised form: 8 November 2012 / Accepted: 8 November 2012 / Published: 13 November 2012 Abstract: Recent developments in studies concerning cell-sized vesicles, such as liposomes with a lipid bilayer and water-in-oil droplets covered by a lipid monolayer, aim to realize the real-world modeling of living cells. Compartmentalization with a membrane boundary is essential for the organization of living systems. Due to the relatively large surface/volume ratio in microconfinement, the membrane interface influences phenomena related to biological functions. In this article, we mainly focus on the following subjects: (i) conformational transition of biopolymers in a confined space; (ii) molecular association on the membrane surface; and (iii) remote control of cell-sized membrane morphology. Keywo