大鼠亚慢性丙烯酰胺中毒神经行为功能及电生理的改变1).pdf

# 278# 2008 8 35 4 Chin OccupM ed, August, 2008, Vol. 35, No. 4 : 1000- 6486( 2008) 04- 0278- 03 张艳青, 姚林, 王广增, 张艳淑 : 观察丙烯酰胺亚慢性染毒对大鼠神经行为功能及电生理变化的影响 30 只雄性 SD大鼠 机 分为 3组(对照组低和高剂量组)每组 10只, 分别以生理氯化钠丙烯酰胺 2040mg/kg 剂量腹腔注射,每周 3次, 共 10 周, 测量大鼠体重热板仪甩尾后肢撑力及神经传导速度等指标 中毒后大鼠体重减轻, 从第 4周开始高剂量 组与对照组比较有统计学意义(P 005), 第 6周高剂量组与低剂量组比较有统计学意义(P 001), 热板仪实验异常 (高剂量组时间延长 57%,低剂量组时间延长 44% ) , 甩尾实验时间延长(高剂量组延长 38% ), 后肢展开距离明显加宽 (高剂量组增加 102%, 低剂量组增加 61% ),胫神经和腓肠神经传导速度降低(感觉神经传导速度降低 32%, 运动神经 传导速度降低 33% ) 丙烯酰胺可引起大鼠的热觉传导异常, 运动神经损伤,传导速度降低 : 丙烯酰胺; 神经行为功能; 电生理学 : R135. 1 : A Changes of neurobehav ior function and electrophysiology in rats exposed to acrylam ide ZHANG anqing, AO Lin, WANG Guangzeng, et al (Dapartment ofPreventiveM edicine, North China CoalM edicalUniversity, Tangshan 063000, China) Abstract: Objective To observe the changes of neurobehavior function and electrophysiology in rats exposed to acrylam ide. M ethods M ale SD rats ( 220~ 250 g) were randomly divided into 3 groups ( 10 rats per group). The rats in group 1were served as the control(NS only) and the other2 groupswere given acrylamide ( 20 or40mg/kg ip, 3 tmi es/week) for 10weeks. Special tests included observations ofnervous sensory to pain, measurements of hindlmi b landing foot splay and electrophysiologi cal changes in rats. Results Theweight of the rats treatedwith acrylamidewere reduced (P 005) after 3weeks of exposure. Comparedw ith the control group, the hind pawlick latency of rats at highdose groupwas significantly prolonged by 57% (P 001), the tailflick lantency at high dose groupwas prolonged by 38%, hindlmi b landing foot splay at lowdose and highdose group were increased by 102% and 61%, respectively (P 005). Between ACR an