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柠檬酸_乙醇法提取紫甘薯花青素工艺的优化.docx

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柠檬酸—乙醇法提取紫甘薯花青素工艺的优化樊 婷 1,何建军 2,陈学玲 2,关健 2,梅新 2(1.华中农业大学楚天学院,武汉 430205;2.湖北省农业科技创新中心农产品加工研究分中心 / 湖北省农业科学院农产品加工与核农技术研究所,武汉430064)摘要:以紫甘 薯(Ipomoes batats L.)为原料,采用 柠檬酸— 乙醇法提取花 青 素,通过单因素及正交试验探讨了提取剂中柠檬酸与乙醇体积比、柠檬酸浓度、液料比、提取温度和 提取时间等 因素对花 青 素提取效 果的影响,优化 提取工艺。 结果表明,各因素对紫薯花 青 素提取的影响由大到小 依次为液料比、柠檬酸浓 度、提取温度、提取时间,优化 的提取工艺条件为柠檬酸与乙醇体积比 10∶1、柠檬酸浓度 70 g / L、 提取 温 度 70 ℃,提取时间 2.0 h,液料比 20∶1(V∶m,mL/g)。关键词:紫甘 薯(Ipomoes batats L.);花 青 素;提取;柠檬酸— 乙醇法中图分类号:S531;Q946.83+6文献标识码:A文章编号:0439-8114(2012)23-5448-04Optimization of Extraction Technology of Anthocanin from Purple Sweet Potato byCitric Acid-Ethanol MethodFAN Ting1,HE Jian-jun2,CHEN Xue-ling2,GUAN Jian2,MEI Xin2(1.Chutian College, Huazhong Agricultural University, Wuhan 430205, China; 2.Agro-Processing Sub-Centers of Hubei Innovation Center of Agricultural Science / Institute for Farm Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China)Abstract: Using purple sweet potato(Ipomoes batats L.) as material, anthocyanin was extracted by citric acid-ethanol method.Single factor tests and orthogonal design were conducted to study the effects of volume ratio of citric acid to ethanol , citric acid concentration, liquid to solid ratio, extraction temperature and extraction time on yield of anthocyanin so as to optimize the extraction technology. The results indicated that the effect of factors on extraction yield of anthocyanin ranking from largest to smallest were liquid to solid ratio, citric acid concentration, extraction temperature, extraction time. The optimal antho- cyanin extraction technology of citric acid -ethanol method from purple sweet potato was the volume ratio of citric acid and ethanol, 10∶1; citric acid concentration, 70 g / L; extraction temperature, 70 ℃; extraction time, 2.0 h; ratio of liquid to sol- id, 20∶1(V∶m,mL/g).Key words: purple sweet potato(Ipo
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