基础研究肾癌细胞系SN12C和786-O无血清培养的干细胞球特性的比较.pdf
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中国肿瘤临床2016年第43卷第23期 Chin J Clin Oncol 2016. Vol. 43. No. 23
肾癌细胞系SN12C和786-O无血清培养的干细胞球特性的比较*
张艳辉① 孙保存①②③ 赵秀兰②③ 刘志勇① 姚欣① 董学易②③
摘要 目的:本研究通过比较SN12C和786-O两株肾癌细胞系中肿瘤干细胞特征的差异性,初步探讨筛选肾癌干细胞更有效的
方法。方法:以无血清培养法培养 SN12C和 786-O细胞,比较其在不同时间形成肿瘤干细胞球的差异;应用流式细胞术分析
SN12C和786-O球体细胞中干细胞标志物的表达情况;利用裸鼠体内成瘤模型检测SN12C和786-O球体细胞成瘤能力。结果:
SN12C较786-O更易形成肿瘤干细胞球且所需时间更短,即786-O在无血清培养的第7天开始形成规则的球体,而SN12C则在第
5天就已形成规则的球体。SN12C和786-O球体细胞中干细胞指标表达量亦有显著性差异,在SN12C球体细胞中CD133、CD44、
Nanog和Oct3/4比例明显高于786-O球体细胞,差异具有统计学意义(P0.05)。裸鼠体内的成瘤能力实验发现SN12C明显强于
786-O球体细胞。结论:SN12C肾癌细胞系通过无血清培养方法富集肿瘤干细胞球明显多于786-O,是获得肿瘤干细胞较好的研
究对象。
关键词 肾癌 肿瘤干细胞 SN12C细胞 786-O细胞
doi:10.3969/j.issn.1000-8179.2016.23.071
Characteristics of stem cell spheres of the renal carcinoma cell lines SN12C and 786-O
grown in serum-free culture medium
Yanhui ZHANG1, Baocun SUN1,2,3, Xiulan ZHAO2,3, Zhiyong LIU1, Xin YAO1, Xueyi DONG2,3
Correspondence to: Baocun SUN; E-mail: baocunsun@
1Department of Patholagy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tian-
jin Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China; 2Department of Patholagy, Tianjin Medical University, Tian-
jin 300070, China; 3Department of Pathology, Tianjin Medical University General Hospital, Tianjin 300020, China
This work was supported by the Tianjin Health Bureau Science and Technology Fund (No. 2014KZ081)
Abstract Objective: This study aims to determine the suitable cell line to be used in isolating cancer stem cells by comparing the
characteristics of tumor stem cells in renal cell carcinoma cell lines SN12C and 786-O. Methods: The rate of sphere formation in SN12C
and 786-O cells was determined in serum-free medium (SFM). The expression levels of CD133, CD44, Nanog, and Oct3/4 were investi-
gated through flow cytometry. Moreover, the tumorigenicity of spheroid cell that originated from SN12C and 786-O cells was investi-
gated in vivo by using a tumor model. Results: The average time of sphere formation
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