侧脑室注射共轭亚油酸（CLA）对大鼠糖脂代谢的影响.doc

侧脑室注射共轭亚油酸（CLA）对大鼠糖脂代谢的影响 ? 544?现代生物医学进展叭v.biomed.net.cnProgressinModemBiomedicine2007VoL.7No..4 侧脑室注射共轭亚油酸(CLA)X~鼠糖脂代谢的影响 曹子鹏王枫向雪松曹瑞张文斌史永亮高双斌 (第四军医大学军事预防医学系营养与食品卫生学教研室西安710032) 摘要目的:观察侧脑室注射共轭亚油酸(Conjugatedfinoleicacid,CLA)对SD大鼠糖脂代谢的影响及其可能的机制.方法:向正常 SD大鼠侧脑室内注射共轭亚油酸(CLA),分别于注射后2,4,8,12,24小时采血,试剂盒法测血糖,胰岛素,瘦素,血甘油S-Jl~,血 胆固醇,血高密度脂蛋白.48小时后处死,分离动物的脂肪(皮下,内脏,肾周,睾周)进行称重,计算体脂比.结果:与对照组相比, 侧脑室注射CLA48小时后,大鼠脂体比,皮下脂肪,睾周脂肪均下降.术后2-12h血糖降低,血清胰岛素浓度也降低,而且持续的 时间较长(48h).侧脑室注射CLA对脂代谢有影响,2h时血清甘油三酯升高,胆固醇降低,4h时高密度脂蛋白升高.结论:共轭亚 油酸能够通过中枢神经系统调节外周糖脂代谢,这可能与其能减轻体重的机制有关. 关键词:共轭亚油酸;中枢神经系统;糖脂代谢;脂解作用 中图分类号:Q591文献标识码:A论文编号:1673—6273(2007)04—0544—04 EffectsofIntracerebroventricularAdministrationofConjugatedLinoleic Acid(CLA)onGlucoseandLipidMetabolisminRats CAOZi-peng,WANGFeng,XIANGXue-song,CAORuiZHANGWen—bin,SHIYong一4iang,GAOShuang一-bin (DepartmentofNutritionandFoodHygene,theFourthMilitaryMedicalUniversity,Xian710032,CHINA) ABSTRACTObjiective:Dietaryconjugatedlinoleicacid(CLA)isbeinginvestigatedforbeneficialeffectsfordisease preventionandtreatment,includingobesity,inavarietyofexperimentalmodds,butthemechanismisnotdear.By IntracerebroventricularadministrationofCLA,ourexperimentwasconductedtoextendtheseobservationstohypothalamusand otherregionswithinthecentralnervoussystemsothatthemechanismoftheactionsofCLAmightbemoreeasilyelucidated. Methods:Fortheexperiment,normalSprague-DawleyratsweredividedintothreegroupsandreceivedICVinjactionsofeither 150nmolCLA,orLAasnon一-conjugatedcontrol,ornormalsaline.Wecollectedthebloodsampleat2,4,8,12,24hoursand triglycerides,totalcholesterol,HDLcholesterol,andgIucoseconcentrationsintheplasmaweremeasuredusingkits.Theratswere dissectedafter48hoursandadiposetissues(subcutaneous,mesenteric.,retroperitoneal,epididyma1)wereremoved.,blotteddry,and immediatelyweighed.Results:Thefat/bodyweight,subcutaneousandretro--peritonealadiposetissuedecreasedafter48hours. Theplasmaucosedecreasedat2-12hoursandinsulinconcentrationalsoreduced.Theplasmatriglyceridesincreasedandtotal cholesteroldecrea