the endocrine disruptor mono-(2-ethylhexyl) phthalate affects the differentiation of human liposarcoma cells (sw 872)的内分泌干扰物mono -邻苯二甲酸二(2-ethylhexyl)影响人类脂肪肉瘤细胞的分化(sw 872).pdf

The Endocrine Disruptor Mono-(2-Ethylhexyl) Phthalate Affects the Differentiation of Human Liposarcoma Cells (SW 872) 1,2 1 1 1 Enrico Campioli , Amani Batarseh , Jiehan Li , Vassilios Papadopoulos * 1 Research Institute of the McGill University Health Center and the Departments of Medicine, Biochemistry, and Pharmacology and Therapeutics, McGill University, ´ ´ Montreal, Quebec, Canada, 2 Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena, Italy Abstract Esters of phthalic acid (phthalates) are largely used in industrial plastics, medical devices, and pharmaceutical formulations. They are easily released from plastics into the environment and can be found in measurable levels in human fluids. Phthalates are agonists for peroxisome proliferator-activated receptors (PPARs), through which they regulate translocator protein (TSPO; 18 kDa) transcription in a tissue-specific manner. TSPO is a drug- and cholesterol-binding protein involved in mitochondrial respiration, steroid formation, and cell proliferation. TSPO has been shown to increase during differentiation and decrease during maturation in mouse adipocytes. The purpose of this study was to establish the effect of mono-(2- ethylhexyl) phthalate (MEHP) on the differentiation of human SW 872 preadipocyte cells, and examine the role of TSPO in the process. After 4 days of treatment with 10 mM MEHP, we observed changes in the transcription of acetyl-CoA carboxylase alpha, adenosine triphosphate citrate lyase, glucose transporters 1 and 4, and the S100 calcium binding protein B, all of which are markers of preadipocyte differentiation. These observed gene expression changes coincided with a