the glycobiome of the rumen bacterium butyrivibrio proteoclasticus b316t highlights adaptation to a polysaccharide-rich environment瘤胃细菌的glycobiome丁酸弧菌属proteoclasticus b316t强调适应polysaccharide-rich环境.pdf

The Glycobiome of the Rumen Bacterium Butyrivibrio proteoclasticus B316T Highlights Adaptation to a Polysaccharide-Rich Environment 1 1 1 1,2¤ 1,3 William J. Kelly , Sinead C. Leahy , Eric Altermann , Carl J. Yeoman , Jonathan C. Dunne , Zhanhao 1 1 1 1 1 1 Kong , Diana M. Pacheco , Dong Li , Samantha J. Noel , Christina D. Moon , Adrian L. Cookson , Graeme T. Attwood1* 1 Rumen Microbial Genomics, AgResearch, Grasslands Research Centre, Palmerston North, New Zealand, 2 Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand, 3 Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand Abstract Determining the role of rumen microbes and their enzymes in plant polysaccharide breakdown is fundamental to understanding digestion and maximising productivity in ruminant animals. Butyrivibrio proteoclasticus B316T is a Gram- positive, butyrate-forming rumen bacterium with a key role in plant polysaccharide degradation. The 4.4Mb genome consists of 4 replicons; a chromosome, a chromid and two megaplasmids. The chromid is the smallest reported for all bacteria, and the first identified from the phylum Firmicutes. B316 devotes a large proportion of its genome to the breakdown and reassembly of complex polysaccharides and has a highly developed glycobiome when compared to other sequenced bacteria. The secretion of a range of polysa