9基因工程抗体和抗体工程.pptx

第五节 抗体工程;C genes code for the constant regions of immunoglobulin protein chains.V gene is sequence coding for the major part of the variable (N-terminal) region of an immunoglobulin chain.;Figure 24.4 Heavy and light chains combine to generate an immunoglobulin with several discrete domains.;Figure 24.4 Heavy and light chains combine to generate an immunoglobulin with several discrete domains.;Table 24.1 Each immunoglobulin family consists of a cluster of V genes linked to its C gene(s).;Figure 24.5 The lambda C gene segment is preceded by a J segment, so that V-J recombination generates a functional lambda light-chain gene.;Figure 24.6 The kappa C gene segment is preceded by multiple J segments in the germ line. V-J joining may recognize any one of the J segments, which is then spliced to the C gene segment during RNA processing.;Figure 24.7 Heavy genes are assembled by sequential joining reactions. First a D segment is joined to a J segment; then a V gene segment is joined to the D segment.;Figure 24.8 The lambda family consists of V gene segments linked to a small number of J-C gene segments.;Figure 24.9 The human and mouse kappa families consist of V gene segments linked to 5 J segments connected to a single C gene segment.;Figure 24.10 A single gene cluster in man contains all the information for heavy-chain gene assembly.;Figure 24.12 Consensus sequences are present in inverted orientation at each pair of recombining sites. One member of each pair has a spacing of 12 bp between its components; the other has 23 bp spacing.;Figure 24.13 Breakage and reunion at consensus sequences generates immunoglobulin genes.;Figure 15.8 Reciprocal recombination between direct repeats excises the material between them; each product of recombination has one copy of the direct repeat.;Figure 15.9 Reciprocal recombination between inverted repeats inverts the region between them.;一、噬菌体抗体库技术的基本方法; 它是在PCR技术和Phage Display的基础上实现的。其过程是把用PCR法得到的抗体基因插入丝状噬菌体的DNA，与噬菌体外壳蛋白的基因相连，在辅助噬菌体的帮