blue light acts as a double-edged sword in regulating sexual development of hypocrea jecorina (trichoderma reesei)蓝光作为一把双刃剑在调节性发展hypocrea jecorina(木霉属reesei).pdf

Blue Light Acts as a Double-Edged Sword in Regulating Sexual Development of Hypocrea jecorina (Trichoderma reesei) 1 1 1 1 1 2 Chia-Ling Chen , Hsiao-Che Kuo , Shu-Yu Tung , Paul Wei-Che Hsu , Chih-Li Wang , Christian Seibel , 2 3 1 Monika Schmoll , Ruey-Shyang Chen *, Ting-Fang Wang * 1 Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, 2 Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria, 3 Department of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan Abstract The industrially important cellulolytic filamentous fungus Trichoderma reesei is the anamorph of the pantropical ascomycete Hypocrea jecorina. H. jecorina CBS999.97 strain undergoes a heterothallic reproductive cycle, and the mating yields fertilized perithecia imbedded in stromata. Asci in the perithecia contain 16 linearly arranged ascospores. Here, we investigated H. jecorina sexual development under different light regimes, and found that visible light was dispensable for sexual development (stroma formation and ascospore discharge). By contrast, constant illumination inhibited stroma formation, and an interruption of the darkness facilitated timely stroma formation in a 12 h/12 h light-dark photoperiod. The results of genetic analyses further revealed that H. jecorina blue-light photoreceptors (BLR1, BLR2) and the photoadaptation protein ENV1 were not essential for sexual development in general. BLR1, BLR2 and ENV1 are orthologues of the conserved Neurospora crassa WC-1, WC-2 and VVD, respectively. Moreover, BLR1 and B