RUNX3基因在前列腺癌细胞迁移、侵袭和血管生成中的作用及分子机制研究的开题报告.docx

RUNX3基因在前列腺癌细胞迁移、侵袭和血管生成中的作用及分子机制研究的开题报告

Title:TheRoleandMolecularMechanismsofRUNX3GeneinProstateCancerCellMigration,InvasionandAngiogenesis

Introduction:

Prostatecancerisoneofthemostcommonmalignanciesinmenworldwide,withitsincidenceandmortalityrateincreasingyearbyyear.Metastasis,invasionandangiogenesisaremajorfeaturesofadvancedprostatecancer,whichcontributetoitspoorprognosis.TheRUNX3gene,encodingatranscriptionfactor,hasbeenidentifiedasanoveltumorsuppressorgeneinvarioushumancancers.PreviousstudieshaveindicatedthatRUNX3geneexpressionisdecreasedinprostatecancertissues,anditsdownregulationisassociatedwithtumorprogressionandmetastasis.However,thepreciserolesandmolecularmechanismsofRUNX3geneinprostatecancercellmigration,invasionandangiogenesisremainlargelyunknown.

Objectives:

1.ToinvestigatetheexpressionlevelsofRUNX3geneinprostatecancertissuesanditscorrelationwithclinicopathologicalparameters.

2.ToexploretheeffectsofRUNX3geneoverexpressionorknockdownonprostatecancercellmigration,invasionandangiogenesisinvitro.

3.ToelucidatetheunderlyingmolecularmechanismsofRUNX3gene-mediatedregulationofprostatecancerprogression.

Methods:

1.QuantitativeRT-PCRandWesternblottingwillbeusedtodetecttheexpressionlevelsofRUNX3geneinprostatecancertissuesandcelllines.

2.CellmigrationandinvasionassayswillbeperformedtoassesstheeffectofRUNX3geneoverexpressionorknockdownonprostatecancercellmotilityandinvasiveness.

3.TubeformationassaywillbeusedtoevaluatetheeffectofRUNX3geneonangiogenesis.

4.Immunofluorescencestainingandco-immunoprecipitationwillbeperformedtoinvestigatethepotentialmolecularmechanismsofRUNX3geneinregulatingprostatecancerprogression.

Expectedoutcomes:

Thisstudywillprovideacomprehensiveunderstandingoftheroleandmolecularmechani