高通量测序技术及其在表观遗传学上的应用.pdf

第24卷第7期 生命科学 Vol. 24, No. 7 2012年7月 Chinese Bulletin of Life Sciences Jul., 2012 文章编号：1004-0374(2012)07-0705-07 ∙ 技术与应用 ∙ 高通量测序技术及其在表观遗传学上的应用 1, 2 * 2 1 3 张际峰 ，王洋 ，汪成润 ，聂刘旺 (1 淮南师范学院生命科学系, 淮南232001; 2 复旦大学生命科学学院, 上海200433; 3 安徽师范大学生命科学学院, 芜湖24 1000) DNA 摘　要： 测序技术是现代生命科学研究的重要工具之一，而高通量测序技术在全基因组的研究中发挥 着越来越重要的作用。简要回溯DNA 测序技术的产生与发展，着重从PCR 扩增测序和单分子测序两个方 面全面描述了高通量测序中众多代表性的技术及直接测序技术，并从DNA 甲基化、组蛋白修饰、非编码 RNA 调控等方面阐述了高通量测序技术在表观遗传学上的运用。 关键词：高通量测序 ；表观遗传学；DNA 甲基化；组蛋白修饰 ；非编码RNA 中图分类号 ：Q34 ；Q755 文献标识码：A High-throughput sequencing technology and its application in epigenetics 1, 2 2 1 3 ZHANG Ji-Feng *, WANG Yang , WANG Cheng-Run , NIE Liu-Wang (1 Department of Life Science, Huainan Normal University, Huainan 232001, China; 2 School of Life Science, Fudan University, Shanghai 200433, China; 3 College of Life Science, Anhui Normal University, Wuhu 241000, China) Abstract: The DNA sequencing technology is one of the most powerful tools in modern life science research, and the high-throughput sequencing technologies play a key role in genomic sequencing of different organisms. Here, we presented a brief review of the invention and development of the DNA sequencing technologies. Furthermore, we described the representative sequencing technologies and their instruments of high-throughput sequencing technologies, which could be divided into two categories, PCR-based sequencing and single molecule sequencing. Then we also provided insights into