status of b-vitamins and homocysteine in diabetic retinopathy association with vitamin-b12 deficiency and hyperhomocysteinemia地位的维生素b和同型半胱氨酸在糖尿病性视网膜病变与维生素b12缺乏症和半胱氨酸.pdf

Status of B-Vitamins and Homocysteine in Diabetic Retinopathy: Association with Vitamin-B12 Deficiency and Hyperhomocysteinemia 1 1 1 1 Alleboena Satyanarayana , Nagalla Balakrishna , Sujatha Pitla , Paduru Yadagiri Reddy , Sivaprasad 1 1 1 2 3 Mudili , Pratti Lopamudra , Palla Suryanarayana , Kalluru Viswanath , Radha Ayyagari , Geereddy Bhanuprakash Reddy1* 1 Biochemistry, National Institute of Nutrition, Hyderabad, India, 2 Pushpagiri Vitreo Retina Institute, Hyderabad, India, 3 Ophthalmology, University of California, San Diego, San Diego, California, United States of America Abstract Diabetic retinopathy (DR) is a common cause of blindness. Although many studies have indicated an association between homocysteine and DR, the results so far have been equivocal. Amongst the many determinants of homocysteine, B-vitamin status was shown to be a major confounding factor, yet very little is known about its relationship to DR. In the present study, we, therefore, investigated the status of B-vitamins and homocysteine in DR. A cross-sectional case–control study was conducted with 100 normal control (CN) subjects and 300 subjects with type-2 diabetes (T2D). Of the 300 subjects with T2D, 200 had retinopathy (DR) and 100 did not (DNR). After a complete ophthalmic examination including fundus fluorescein angiography, the clinical profile and the blood levels of all B-vitamins and homocysteine were analyzed. While mean plasma homocysteine levels were found to be higher in T2D patients compared with CN subjects, homocysteine levels were particularly high in the DR group. There were no group differences in the blood le