细胞因子联合纹状体条件培养液定向诱导间充质干细胞体外分化为多巴胺能神经元.pdf

中国组织工程研究与临床康复 第 2春 筹钳 期 2008 lO一2l出版 JournalofClinicalRehabilitativeTissueEngineeringResearch 0ctober21 2008 If_j2No．43 囊 襞 基础医学 细胞因子联合纹状体条件培养液定向诱导间充质干细胞体外分化为 多巴胺能神经元☆ 李 府’，马丽霞’，张乐玲 ，郑立波’，陈颖杰 ，吴 镇 ，王世富 Cytokinescombinedwithstriatalconditionedmedium toinducedifferentiationofmesenchymalstem cells intodopaminergicneurons／nvitro LiFu ，MaLi-xia’ZhangLe．1ing’ZhengLi—bo’ChenYing-jie，WuZhen，WangShi-fu’ ， ， ， Abstract BACKGR0UND：Inductionrateofbonemarrowmesenchymalstem cells(BMSCs)todifferentiateintodopaminergicneuronsfn PediatricReesarch vitroisnotsatisfactory． Institute，Qilu children’sHospitalof OBJECTIVE：Toexplorethepossibilityofbasicfibroblastgrowhtfactor，epidermalgrowhtfactorcombinedwithstriatal ShandongUniversity, conditionedmedium topromotedirectionaldifierentiationofMSCsintodopaminergicneurons． Jinan 25o022． DESIGN．TIMEANDSETTING：Cellcontrolobservation．TheexperimentwasperformedathteHematologyLaboratoryofQilu ShandongProvnice， Children’sHospitalandSecondHospitalofShandongUniversityfrom July2006toDecember2007． Chnia；Hematology Laboratory,Second M ATERIALS：HealthyadultWistarratswereselectedforMSCsisolation．andnewbornW istarratswereselectedforpreparation