conjugation of functionalized spions with transferrin for targeting and imaging brain glial tumors in rat model接合与转铁蛋白功能化spions瞄准和成像在老鼠大脑神经胶质肿瘤模型.pdf

Conjugation of Functionalized SPIONs with Transferrin for Targeting and Imaging Brain Glial Tumors in Rat Model 1. 2. 1 1 1 1 3 Weili Jiang , Hui Xie , Devina Ghoorah , Yalei Shang , Haojun Shi , Fang Liu , Xiangliang Yang *, Haibo Xu1* 1 Radiology Department of Union Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, People’s Republic of China, 2 Department of Information Processing, China Patent Information Center, Wuhan, People’s Republic of China, 3 College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, People’s Republic of China Abstract Currently, effective and specific diagnostic imaging of brain glioma is a major challenge. Nanomedicine plays an essential role by delivering the contrast agent in a targeted manner to specific tumor cells, leading to improvement in accurate diagnosis by good visualization and specific demonstration of tumor cells. This study investigated the preparation and characterization of a targeted MR contrast agent, transferrin-conjugated superparamagnetic iron oxide nanoparticles (Tf- SPIONs), for brain glioma detection. MR imaging showed the obvious contrast change of brain glioma before and after administration of Tf-SPIONs in C6 glioma rat model in vivo on T2 weighted imaging. Significant contrast enhancement of brain glioma could still be clearly seen even 48 h post injection, due to the retention of Tf-SPIONs in cytoplasm of tumor cells which was proved by Prussian blue staining. Thus, these results suggest that Tf-SPIONs could be a potential targeting MR contrast agent for the brain glioma. Citation: Jiang W, Xie H, Ghoorah D, Shang Y, Shi H, et al. (2012) Conjugatio