阿托伐他汀对糖尿病大鼠视网膜NF-κB表达影响的实验研究-毕业论文（设计）.doc

阿托伐他汀对糖尿病大鼠视网膜NF-κB表达影响的实验研究 摘 要 目的：探讨他汀类药物对糖尿病大鼠视网膜NF-κB表达的影响及其可能机制。 方法：雄性SD大鼠42只，随机抽28只腹腔注射链脲佐菌素65mg/kg建立糖尿病模型，余14只为正常对照组。糖尿病大鼠成模后，随机分成两组，糖尿病非药物干预组及阿托伐他汀干预组。干预组予阿托伐他汀（2mg/kg/day）灌胃，对照组及糖尿病非药物干预组给予等量饮用水。大鼠分别于3个月，6个月时按比例处死。取一眼视网膜组织抽提RNA，另一眼固定后做免疫组织化学观察。应用RT-PCR方法扩增NF-κB基因，比较3组大鼠的NF-κB在基因水平上的表达差异。应用免疫组织化学方法来观察NF-κB在眼内的分布，并比较3组大鼠的差异。结果：PCR结果示糖尿病大鼠视网膜中NF-κB在基因水平上比正常大鼠明显增高（P＜0.05），药物干预的大鼠，NF-κB的表达比糖尿病非药物干预组明显减少（P＜0.05）。免疫组织化学方法证明在视网膜上NF-κB主要分布在血管层，糖尿病大鼠视网膜中NF-κB阳性的细胞比正常大鼠明显增多（P＜0.05），且着色较深。阿托伐他汀干预组的大鼠中，NF-κB阳性的细胞比糖尿病大鼠明显减少（P＜0.05），且着色较浅。结论：阿托伐他汀能降低糖尿病大鼠视网膜中NF-κB mRNA及NF-κB蛋白质的表达，减缓视网膜病变进展。 关键词：阿托伐他汀 ；糖尿病视网膜病变；NF-κB 作者单位：116001 大连大学附属中山医院内分泌科（魏薇、于晓静、王晓梅、隋淼 李湘）；大连大学附属中山医院病理科（朱瑞萍） 通讯作者：于晓静 邮编：yxj_y@ 大连市市卫生局资助课题，【2009】67 Experimental study of influence of Atrovastatin on expression of NF-κB in Retionpathy of Diabetic Rats Wei- wei, Yu Xiao-jing , WANG Xiao-mei, et al. Endocrinology Department of Afiliated zhong shan Hospital of Da Lian University, Dalian 116001,China Correspongding author: Yu Xiao-jing Abstract Objective: To explore the effects and mechanism of atrovastatin on expression of the NF-κB on retionpathy of diabetic rats. Methods : A total of 42 male SD rats were randomly taken out 28 to make diabetes model by induced injection of 65mg streptozotocin (STZ)into enterocoelia,the rest was normal control group . After the model maded , atrovastatin(2mg/kg/d) were given the treated group ,and normal control group and diabetic rats without treatment group was given equivalent water.After 3 and 6 months,the rats were killed .Isolated total RNA of the retina from one eye for each rat ,and the other eye was prepared for the examination of immunohistochemistry. Determinated the NF-κB mRNA expression amoug three groups by the method of RT-PCR. Observed the distribution of the NF-κB in the retina, and compared the diference of three group. Results: PCR shows that NF-κB mRNA was increased in the retina of diabetic compared with control rats（P＜0.05）. D