三氧化二砷纳米粒的制备及对4种肿瘤细胞的抑制作用.pdf

·论 著 · 三氧化二砷纳米粒的制备及对4 种肿瘤细胞的抑制作用  于莲，匡宇明，杨金儒，刘洋，周彤，杨佳蓉(佳木斯大学药学院，黑龙江省高校生物药制剂重点实验室，黑龙江 佳木斯 154007) 摘要：目的 采用星点设计优化处方，制备三氧化二砷固体脂质纳米粒并探讨其对4 种肿瘤细胞的抑制作用。方法 采 用乳化超声法制备三氧化二砷固体脂质纳米粒。采用MTT 法考察三氧化二砷固体脂质纳米粒对4 种肿瘤细胞的体外抑制 作用。结果 筛选的最优处方为PEG-单硬脂酸甘油酯用量0.11 g ，大豆卵磷脂的用量0.18 g 。平均粒径为131.54 nm，包 封率73.46% ，载药量为1.07%。不同浓度三氧化二砷固体脂质纳米粒对4 种细胞均有抑制增殖作用。结论 采用乳化超 声法制得的三氧化二砷固体脂质纳米粒具有较好的稳定性，粒子分布均匀，符合制剂学要求。携带阳离子三氧化二砷固 体脂质纳米粒组抑制作用最强。 关键词：三氧化二砷；固体脂质纳米粒；乳化超声法；MTT ；肿瘤抑制作用 中图分类号：R284.1 ；R917 文献标志码：B 文章编号：1007-7693(2013)07-0701-07 Preparation of Arsenic Trioxide Nanoparticles the Cytotoxicity against Four Tumor Cells YU Lian, KUANG Yuming, YANG Jinru, LIU Yang, ZHOU Tong, YANG Jiarong(Key Laboratory of Bio- pharmaceutical Preparations of Ji amusi University College of Pharmacy University of Heilongjiang, Jiamusi University, Jiamusi 154007, China) ABSTRACT: OBJECTIVE To prepared arsenic trioxide solid liguid nanoparticles with central composite design and study the inhibitory effect of arsenic trioxide solid liqid nanoparticles on four kinds of tumor cells. METHODS Arsenic trioxide solid lipid nanoparticles were prepared by emulsion-ultrasonic dispersion method. And MTT experiment was used to investigate the inhibition of four tumor cells. RESULTS The optimal formulation was screened as monostearin 0.11 g and soy lecithin 0.18 g. Average size of nanoparticles was 131.54 nm. Encapsulation efficiency was 73.46% and drug loading was 1.07%. The proliferations of four cells were inhibited by different concentrations of arsenic trioxide. And nanoparticles further induced the apoptosis of cells. CONCLUSION Arsenic trioxide solid lipid nanoparticles prepared by emulsion-ultrasonic dispersion method owned well stability and uniformity of particle distribution which meets pharmaceutical requirement. The inhibitory effect