substantial alterations of the cutaneous bacterial biota in psoriatic lesions实质性改变的皮肤细菌生物银屑病病变.pdf

Substantial Alterations of the Cutaneous Bacterial Biota in Psoriatic Lesions 1 1,2 3 1,4,6 1,5,6 Zhan Gao , Chi-hong Tseng , Bruce E. Strober , Zhiheng Pei , Martin J. Blaser * 1 Department of Medicine, New York University School of Medicine, New York, New York, United States of America, 2 Department of Environmental Medicine, New York University School of Medicine, New York, New York, United States of America, 3 Department of Dermatology, New York University School of Medicine, New York, New York, United States of America, 4 Department of Pathology, New York University School of Medicine, New York, New York, United States of America, 5 Department of Microbiology, New York University School of Medicine, New York, New York, United States of America, 6 New York Harbor Veterans Affairs Medical Center, New York, New York, United States of America Abstract For psoriasis, an idiopathic inflammatory disorder of the skin, the microbial biota has not been defined using cultivation- independent methods. We used broad-range 16S rDNA PCR for archaea and bacteria to examine the microbiota of normal and psoriatic skin. From 6 patients, 19 cutaneous samples (13 from diseased skin and 6 from normal skin) were obtained. Extracted DNA was subjected to the broad range PCR, and 1,925 cloned products were compared with 2,038 products previously reported from healthy persons. Using 98% sequence identity as a species boundary, 1,841 (95.6%) clones were similar to known bacterial 16S rDNA, representing 6 phyla, 86 genera, or 189 species-level operational taxonomic unit (SLOTU); 84 (4.4%) clones with ,98% identity probably represented novel species. The most abundant and diverse phylum populating the psoriatic