apoptosis-like death in bacteria induced by hamlet, a human milk lipid-protein complexapoptosis-like死亡细菌引起的哈姆雷特,母乳lipid-protein复杂.pdf

Apoptosis-Like Death in Bacteria Induced by HAMLET, a Human Milk Lipid-Protein Complex 1,2,3 1 4 4,5 Anders P. Hakansson *, Hazeline Roche-Hakansson , Ann-Kristin Mossberg , Catharina Svanborg 1 Department of Microbiology and Immunology, University at Buffalo, State University of New York, Buffalo, New York, United States of America, 2 The Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, State University of New York, Buffalo, New York, United States of America, 3 New York State Center of Excellence in Bioinformatics and Life Sciences, Buffalo, New York, United States of America, 4 The Section of Microbiology, Immunology and Glycobiology (MIG), Department of Laboratory Medicine, Lund University, Lund, Sweden, 5 Singapore Immunology Network, A*STAR, Singapore, Singapore Abstract Background: Apoptosis is the primary means for eliminating unwanted cells in multicellular organisms in order to preserve tissue homeostasis and function. It is characterized by distinct changes in the morphology of the dying cell that are orchestrated by a series of discrete biochemical events. Although there is evidence of primitive forms of programmed cell death also in prokaryotes, no information is available to suggest that prokaryotic death displays mechanistic similarities to the highly regulated programmed death of eukaryotic cells. In this study we compared the characteristics of tumor and bacterial cell death induced by HAMLET, a human milk complex of alpha-lactalbumin and oleic acid. Methodology/Principal Findings: We show that HAMLET-treated bacteria undergo cell death with mechanistic and morphologic similarities