生物信息学-课堂练习生物信息学蛋白质序列分析-课堂练习.doc

生物信息学蛋白质序列分析-课堂练习ZNF395, 全称为Zinc Finger Protein395, 又被称为PBF，PRF1，DBP2，PRF-1，Si-1-8-14或DKFZp434K1210。其氨基酸序列为MASVLSRRLGKRSLLGARVLGPSASEGPSAAPPSEPLLEGAAPQPFTTSDDTPCQEQPKEVLKAPSTSGLQQVAFQPGQKVYVWYGGQECTGLVEQHSWMEGQVTVWLLEQKLQVCCRVEEVWLAELQGPCPQAPPLEPGAQALAYRPVSRNIDVPKRKSDAVEMDEMMAAMVLTSLSCSPVVQSPPGTEANFSASRAACDPWKESGDISDSGSSTTSGHWSGSSGVSTPSPPHPQASPKYLGDAFGSPQTDHGFETDPDPFLLDEPAPRKRKNSVKVMYKCLWPNCGKVLRSIVGIKRHVKALHLGDTVDSDQFKREEDFYYTEVQLKEESAAAAAAAAAGTPVPGTPTSEPAPTPSMTGLPLSALPPPLHKAQSSGPEHPGPESSLPSGALSKSAPGSFWHIQADHAYQALPSFQIPVSPHIYTSVSWAAAPSAACSLSPVRSRSLSFSEPQQPAPAMKSHLIVTSPPRAQSGARKARGEAKKCRKVYGIEHRDQWCTACRWKKACQRFLDMASVLSRRLGKRSLLGARVLGPSASEGPSAAPPSEPLLEGAAPQPFTTSDDTPCQEQPKEVLKAPSTSGLQQVAFQPGQKVYVWYGGQECTGLVEQHSWMEGQVTVWLLEQKLQVCCRVEEVWLAELQGPCPQAPPLEPGAQALAYRPVSRNIDVPKRKSDAVEMDEMMAAMVLTSLSCSPVVQSPPGTEANFSASRAACDPWKESGDISDSGSSTTSGHWSGSSGVSTPSPPHPQASPKYLGDAFGSPQTDHGFETDPDPFLLDEPAPRKRKNSVKVMYKCLWPNCGKVLRSIVGIKRHVKALHLGDTVDSDQFKREEDFYYTEVQLKEESAAAAAAAAAGTPVPGTPTSEPAPTPSMTGLPLSALPPPLHKAQSSGPEHPGPESSLPSGALSKSAPGSFWHIQADHAYQALPSFQIPVSPHIYTSVSWAAAPSAACSLSPVRSRSLSFSEPQQPAPAMKSHLIVTSPPRAQSGARKARGEAKKCRKVYGIEHRDQWCTACRWKKACQRFLDLength=513 Weight= 54939 Da结构域分析：http://www.expasy.ch/prosite/(一)分析蛋白质的一级结构分析蛋白质的pI、Mw、氨基酸组成:Tools and software packages------Identification and characterization-----ProtParam HYPERLINK http://www.expasy.ch/tools/protparam.html http://www.expasy.ch/tools/protparam.html分析蛋白质的疏水性:Primary structure analysis-----ProtScale HYPERLINK http://www.expasy.ch/tools/protscale.html http://www.expasy.ch/tools/protscale.html分析蛋白质的重复序列:Primary structure analysis-----REP HYPERLINK http://www.embl-heidelberg.de/~andrade/papers/rep/search.html http://www.embl-heidelberg.de/~andrade/papers/rep/search.html(二)分析蛋白质的二级结构预测蛋白质的?－螺旋和?－折叠结构:Secondary structure prediction-----nnPredict HYPERLINK /~nomi/nnpredict.html /~nomi/nnpredict.html蛋白质的其它二级结构:Secondary structure prediction-----SOPMA(三)分析蛋白质的三级结构molecular modeling:“tertiary structure prediction ”栏目选择选择一个分析工具，email服务(四)分析