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阳离子交换树脂提取拐枣七中总生物碱的工艺研究 韩燕霞，郭增军* ，张卉，韩玲，许文明，李囡(西安交通大学医学院，西安 710061) 摘要：目的 研究用阳离子交换树脂提取拐枣七中总生物碱的工艺条件。方法 以总生物碱的吸附量、解吸率和其中盐 酸小檗碱的含量为考察指标，对 5 种不同型号的阳离子交换树脂进行评价，并考察最佳工艺参数。结果 最佳提取工艺 是：拐枣七药材粉碎成粗粉，加入 10 倍量的70%乙醇，浸泡2 h ，加热回流提取3 次，每次1 h。C008 型阳离子交换树 脂为最佳吸附树脂，其最大吸附量为 0.65 g·g1 ，4% 氨水乙醇为洗脱溶剂，洗脱率为 13.81%。结论 C008 型阳离子交 换树脂可用于提取拐枣七中总生物碱，该方法简便可行。 关键词：拐枣七；总生物碱；阳离子交换树脂；工艺研究 中图分类号：R284.2 文献标志码：A 文章编号：1007-7693(2012)07-0624-04 作者简介：韩燕霞，女，硕士生 Tel:E-mail: hanxia132546@163.com *通信作者：郭增军，男，博士，教授 Tel: (029E-mail: guozj@ ·624 · Chin JMAP, 2012 July, Vol.29 No.7 中国现代应用药学2012 年7 月第29 卷第7 期 Study on Extraction Technology of Alkaloids from Hylomecon Japonica by Cation Exchange Resins * HAN Yanxia, GUO Zengjun , ZHANG Hui, HAN Ling, XU Wenming, LI Nan(School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China) ABSTRACT: OBJECTIVE To obtain the optimal conditions for separating the alkaloids from the extract of Hylomecon japonica by selecting appropriate cation exchange resins. METHODS Five types of cation exchange resins were evaluated in separating efficiency with measuring the adsorption ratio and eluting ratio of total alkaloids as indices, and the content of berberine hydrochloride from Hylomecon japonica was determined as an index by HPLC to choose the optimal technological parameters. RESULTS The optimal result of extraction was obtained as Hylomecon japonica shattered into thick powder, extracted with ten times 70% alcohol at room temperature for 2 hours and refluxing and extracting for 1 h (totally extracting for 3 times). C008 cation exchange resin had the good ability of absorbing the total alkaloid from Hylomecon japonica , and each gram of C008 cation exchange resin could absorb 0.65 g of the total alkaloids, and the desorption ratio was 13.81%. CONC