葛根素对重症急性胰腺炎肺损伤保护作用.doc

葛根素对重症急性胰腺炎肺损伤保护作用[摘要] 目的:探讨葛根素对重症急性胰腺炎(SAP)肺损伤的保护作用。方法:经胆胰管逆行注射5%牛磺胆酸钠制作SAP模型,观察各组大鼠血清和肺组织中磷脂酶A2(PLA2)、肿瘤坏死因子(TNF-α)、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)水平;光镜下观察大鼠肺组织的病理变化。结果:葛根素能减轻SAP大鼠胰腺及肺组织的病理性损伤,降低血清和肺组织中PLA2活性及TNF-α、IL-1、IL-6水平。结论:葛根素能有效抑制SAP时增高的PLA2活性,抑制TNF-α的活化,下调多种细胞因子的表达,减轻肺组织损伤,是一种很有效的治疗药物。 [关键词] 葛根素;胰腺炎;肺损伤;肿瘤坏死因子 [中图分类号] R657.5+1[文献标识码]A [文章编号]1673-7210(2010)04(c)-024-02 The protection of Puerarin on severe acute pancreatitis with lung injury CHEN Bo (Laboratory of Physiology Science, Dongguan Branch of Guangdong Medical College, Dongguan 523808, China) [Abstract] Objective: To explore the protective effect of Puerarin in lung injury of severe acute pancreatitis (SAP). Methods: The model of SAP was performed by injecting 5% Sodium Taurocholate. The changes of PLA2 activity, TNF-α, IL-1 and IL-6 levels of serum and lung tissues were observed. Morphologic changes of lung was observed under light microscopy. Results: Puerarin could reduce the pathological injury of pancreas and lung of SAP rats, and decreased the PLA2 activity and TNF-α, IL-1, IL-6 levels of serum and lung tissues. Conclusion: Puerarin can effectively inhibit the PLA2 activity of SAP, inhibit the activation of TNF-α, and downregulate the expression of multiple factors, reduce lung injury, and it is an effective drug. [Key words] Puerarin; Pancreatitis; Lung injury; TNF-α 重症急性胰腺炎(severe acute pancreatitis,SAP)是一种既有胰腺本身病变,又极易并发远隔脏器损害的急腹症,其中肺损伤是SAP早期常见的并发症和死亡原因,其死亡率高达50%,但其预防和治疗缺少有效的药物,因而一直是临床重点难题之一[1]。葛根素对大鼠SAP有保护作用[2],对大鼠急性肺损伤也有保护作用[3],但对SAP所致的肺损伤有无作用报道较少。本研究旨在观察葛根素对SAP肺损伤大鼠的保护作用。 1 材料与方法 1.1 材料 健康Wistar大鼠,由广东医学院实验动物中心提供;牛磺胆酸钠、卵磷脂为Sigma公司产品;葛根素注射液(Puerarin Injection,PI)为浙江康恩贝制药股份有限公司生产,规格2 ml/0.1 g,批号051201;肿瘤坏死因子(TNF-α)、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)的ELISA试剂盒为TPI公司产品;Anthos 2010型酶标仪。 1.2 大鼠SAP模型的建立 大鼠术前12 h禁食、禁水,以20%氨基甲酸乙酯1 mg/kg腹腔注射麻醉,无菌条件下经腹壁正中切口入腹,除假手术组只翻动胰腺后关腹外,其余两组均在主胰管内逆行插管,注入5%牛磺胆酸钠10 ml/g后结扎主胰管,缝合腹壁。 1.3 实验分组 Wistar大鼠30只,随机分为假手术组(10只)、模型组(10只)、葛根素组(